Protein Extraction
Alkaline Extraction
| Instrument(s) | Purpose | Photo |
|---|---|---|
| Oakton pH 700 meter | To adjust pH to alkaline extraction and isoelectric precipitation conditions |
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| ThermoScientific SnakeSkin dialysis tubing | To purify extracted proteins of salts via reverse osmosis |
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| Beckman centrifuges | To separate soluble and insoluble fractions during alkaline extractions |
|
Ultrafiltration Assisted Salt Extraction
| Instrument(s) | Purpose | Photo |
|---|---|---|
| Sartorius Vivaflow 200 membrane ultrafiltration units | To remove excess salt added during salt extraction via ultrafiltration and diafiltration |
|
| ThermoScientific SnakeSkin dialysis tubing | To purify extracted proteins of salts via reverse osmosis |
|
| Beckman centrifuges | To separate soluble and insoluble fractions during salt extractions |
|
Enzyme-Assisted Extraction
| Instrument(s) | Purpose | Photo |
|---|---|---|
| Oakton pH 700 meter | To adjust pH for optimal enzymatic activity |
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| Water bath with HAAKE DC 10 heat exchanger | To hold protein solutions at optimal temperature to maximize enzymatic activity |
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| Precision microprocessor controlled 280 series water bath | To heat protein solutions to inactivate enzymes following enzyme-assisted protein extraction |
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Pilot Plant Scale Extraction
All of the above protein extraction methods can also be performed at pilot plant scale.