Protein Extraction
Alkaline Extraction
| Instrument(s) | Purpose | Photo |
|---|---|---|
Oakton pH 700 meter |
To adjust pH to alkaline extraction and isoelectric precipitation conditions | 
|
ThermoScientific SnakeSkin dialysis tubing |
To purify extracted proteins of salts via reverse osmosis | 
|
Beckman centrifuges |
To separate soluble and insoluble fractions during alkaline extractions | 
|
Ultrafiltration Assisted Salt Extraction
| Instrument(s) | Purpose | Photo |
|---|---|---|
Sartorius Vivaflow 200 membrane ultrafiltration units |
To remove excess salt added during salt extraction via ultrafiltration and diafiltration | 
|
ThermoScientific SnakeSkin dialysis tubing |
To purify extracted proteins of salts via reverse osmosis | 
|
Beckman centrifuges |
To separate soluble and insoluble fractions during salt extractions |
|
Enzyme-Assisted Extraction
| Instrument(s) | Purpose | Photo |
|---|---|---|
Oakton pH 700 meter |
To adjust pH for optimal enzymatic activity |
|
Water bath with HAAKE DC 10 heat exchanger |
To hold protein solutions at optimal temperature to maximize enzymatic activity | 
|
Precision microprocessor controlled 280 series water bath |
To heat protein solutions to inactivate enzymes following enzyme-assisted protein extraction | 
|
Pilot Plant Scale Extraction
All of the above protein extraction methods can also be performed at pilot plant scale.