Capabilities - Protein Modification

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Protein Modification

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Degree of Hydrolysis

Instrument(s)PurposePhoto
Mettler Toledo DL22 pH Stat Titrator Food and Beverage Analyzer

To hydrolyze proteins in a controlled manner by regulating pH and temperature

To measure the degree of hydrolysis

Image showing a Mettler Toledo DL22 pH Stat Titrator

Cold Atmospheric Plasma Treatment

Instrument(s)PurposePhoto
Two-dimensional dielectric barrier discharge setupTo alter protein structure with non-thermal treatment to improve protein functionality
A schematic of a 2D-DBD apparatus used for generate cold atmospheric plasma

Maillard-induced Glycation

Instrument(s)PurposePhoto
Memmert HPP 260 humidity and temperature-controlled shelf-life chamberTo initiate the Maillard reaction under controlled temperature, humidity, air composition, and light intensity
Image showing a Memmert HPP 260 humidity chamber
Minolta Chroma MeterTo determine L*a*b* values of protein ingredients before and after Maillard-induced glycation to observe color changes contributed by the Maillard reaction
Image showing a Minolta Chroma Meter
Biotek Synergy HTTo measure percent free amino groups following Maillard-induced glycation via o-phthaldialdehyde (OPA) and Bicinchoninic Acid (BCA) chemical assays
Image showing a BioTek Synergy HT microplate reader unit
Densitometer to scan gels and determine protein band densities (BioRad)To determine the protein profile under reducing and non-reducing conditions following Maillard-induce glycation
Image showing a Bio-Rad unit used for imaging gels
Hydrophobic interaction chromatographyTo purify protein of unreacted carbohydrates following Maillard-induced glycation
Image showing a hydrophobic interaction chromatography unit and column