Analytical Instrumentation and Research Capabilities


Analytical Instrumentation

PPIC offers the following analytical instruments:

  • Equipment for preparative chromatography including columns, UV monitor, and fraction collectors
  • Several HPLC systems (Shimadzu), analytical and semi-preparative with PDA detector
  • LC/MS system (Shimadzu) (quadrupole equipped with electrospray ionization source (Waters))
  • EI/CI, ESI, MALDI and high resolution mass spectrometers access through the proteomic center
  • Gas chromatography (GC) and gas chromatography-olfactometry (GCO) Mass Spectrometry
  • Several GC/MS systems (Agilent and Hewlett Packard)
  • Differential scanning calorimetry (DSC) (Mettler Toledo)
  • Temperature controlled microplate reader and plate washer (BioTek Synergy HT and Stat Fax 2600)
  • Humidity and temperature controlled chambers (Memmert HPP 260)
  • Ultrafiltration units
  • Nitrogen combustion system for protein analysis (Leco)
  • Zeta potential and particle size analysis (Malvern Zetasizer)
  • Horiba laser scattering particle size distribution analyzer (LA-960 series)
  • FTIR
  • Almega Raman microscope using SERS (Surface Enhanced Raman Spectroscopy) and ExamineR Raman spectrometer for protein denaturation and characterization studies
  • Densitometer to scan gels and determine protein band densities (BioRad)
  • Cryo-scanning electron microscopy and various microscopy instrumentation accessible through the spectroscopy and microscopy center
  • Several UV/Visible scanning spectrophotometers (with temperature regulated cells and enzyme mechanism software) (Beckman Coulter DU 800)
  • Electrophoresis and isoelectric focusing equipment, 1D and 2D units (BioRad)
  • Western Blot unit
  • Benchtop homogenizers (Silogex D500, IKA RW 20, GEA Niro Soavi Panda)
  • pH Stat titrator (Mettler Toledo DL22 Food and Beverage Analyzer)
  • Pilot scale spray dryer (Buchi)
  • Equipment for analyzing powder properties (Mercury Scientific Evolution and Revolution, and Flodex)
  • Texture analyzer (TA.XT Plus)
  • Clevenger distillation
  • Freeze dryer (VirTis)
  • Centrifuges (Ultra, medium speed, and micro) (Beckman and Eppendorf) 
  • Rotovap with condenser (Buchi R124)
  • Ultra low temperature freezers and walk-in cooler

Research Facilities and Centers

Apart from having well-equipped laboratories, the PPIC researchers will have access to several facilities:

Equipped for protein isolation, purification, and application prototyping 

  • Jacketed mixing vessels - 100, 150, 200 gallon
  • Heat exchangers - 25 gal/min
  • Horizontal decanter - 1 gal/min
  • Disk stack centrifuge - 3 L/min
  • Membrane filtration - micro, ultra, dia, nano, RO
  • Evaporator - 80 lb/hr removal
  • Spray dryer - 5 gal feed/hr
  • Agglomerator - 1 kg batch
  • Freeze dryer - (3) 1 L trays
  • Homogenizers - 1 and 2 stage, 4000 psi
  • Extruder - 50 kg feed/hr 
  • Fluidized bed dryer - batch process or continuous feed from extruder
  • Microthermix UHT beverage processing line - 1 L/min
  • Colloid mill
  • NIR for compositional analysis