Analytical Instrumentation
PPIC offers the following analytical instruments:
- Equipment for preparative chromatography including columns, UV monitor, and fraction collectors
- Several HPLC systems (Shimadzu), analytical and semi-preparative with PDA detector
- LC/MS system (Shimadzu) (quadrupole equipped with electrospray ionization source (Waters))
- EI/CI, ESI, MALDI and high resolution mass spectrometers access through the proteomic center
- Gas chromatography (GC) and gas chromatography-olfactometry (GCO) Mass Spectrometry
- Several GC/MS systems (Agilent and Hewlett Packard)
- Differential scanning calorimetry (DSC) (Mettler Toledo)
- Temperature controlled microplate reader and plate washer (BioTek Synergy HT and Stat Fax 2600)
- Humidity and temperature controlled chambers (Memmert HPP 260)
- Ultrafiltration units
- Nitrogen combustion system for protein analysis (Leco)
- Zeta potential and particle size analysis (Malvern Zetasizer)
- Horiba laser scattering particle size distribution analyzer (LA-960 series)
- FTIR
- Almega Raman microscope using SERS (Surface Enhanced Raman Spectroscopy) and ExamineR Raman spectrometer for protein denaturation and characterization studies
- Densitometer to scan gels and determine protein band densities (BioRad)
- Cryo-scanning electron microscopy and various microscopy instrumentation accessible through the spectroscopy and microscopy center
- Several UV/Visible scanning spectrophotometers (with temperature regulated cells and enzyme mechanism software) (Beckman Coulter DU 800)
- Electrophoresis and isoelectric focusing equipment, 1D and 2D units (BioRad)
- Western Blot unit
- Benchtop homogenizers (Silogex D500, IKA RW 20, GEA Niro Soavi Panda)
- pH Stat titrator (Mettler Toledo DL22 Food and Beverage Analyzer)
- Pilot scale spray dryer (Buchi)
- Equipment for analyzing powder properties (Mercury Scientific Evolution and Revolution, and Flodex)
- Texture analyzer (TA.XT Plus)
- Clevenger distillation
- Freeze dryer (VirTis)
- Centrifuges (Ultra, medium speed, and micro) (Beckman and Eppendorf)
- Rotovap with condenser (Buchi R124)
- Ultra low temperature freezers and walk-in cooler
Research Facilities and Centers
Apart from having well-equipped laboratories, the PPIC researchers will have access to several facilities:
Equipped for protein isolation, purification, and application prototyping
- Jacketed mixing vessels - 100, 150, 200 gallon
- Heat exchangers - 25 gal/min
- Horizontal decanter - 1 gal/min
- Disk stack centrifuge - 3 L/min
- Membrane filtration - micro, ultra, dia, nano, RO
- Evaporator - 80 lb/hr removal
- Spray dryer - 5 gal feed/hr
- Agglomerator - 1 kg batch
- Freeze dryer - (3) 1 L trays
- Homogenizers - 1 and 2 stage, 4000 psi
- Extruder - 50 kg feed/hr
- Fluidized bed dryer - batch process or continuous feed from extruder
- Microthermix UHT beverage processing line - 1 L/min
- Colloid mill
- NIR for compositional analysis
