Sample Preparation and Compositional Analysis

Defatting

• Gred S. Carver Press
• Solvent Defatting

To remove fat prior to protein extraction

Milling

• Fritsch Pulversiette ball mill
• UDY cyclone sample mill

To reduce particle size to improve protein extraction

Freeze Drying

• Harvest Right Tray freeze dryer
• Flask freeze dryers (Thermo Electron Corp, LABCONCO, VIRTIS)

To remove moisture through sublimation

Evaporation

• Clevenger distillation
• BuCHI R124 RotoVapor with condenser

To concentrate a sample prior to further assays

Protein Content

• LECO FP 828
• BuCHI Kjeldahl with digestion, scrubber, and distillation unit

Ash Content

• Thermo Scientific Thermolyne muffle furnace

Moisture Content

• Lab-Line Squaroid Duo-Vac moisture oven
• Karl Fischer moisture analyzer

Water Activity

• AquaLab CX-2 water activity meter

Fat Content

• Mojonnier Bros Co. Mojonnier unit

Starch and Fiber Content

• Megazyme Inc. kits: total starch, total dietary fiber, starch damage & amylopectin:amylase

Identification of Fiber Components

• HPAEC Analysis (High Performance Anion-Exchange Chromatography) with a Pulsed Amperometric Detector